Remodeling of Human Sperm Chromatin Mediated by Nucleoplasmin from Amphibian Eggs. (human sperm/chromatin remodeling/protamine removal/nucleoplasmin)
نویسندگان
چکیده
منابع مشابه
Function of homo- and hetero-oligomers of human nucleoplasmin/nucleophosmin family proteins NPM1, NPM2 and NPM3 during sperm chromatin remodeling
Sperm chromatin remodeling after oocyte entry is the essential step that initiates embryogenesis. This reaction involves the removal of sperm-specific basic proteins and chromatin assembly with histones. In mammals, three nucleoplasmin/nucleophosmin (NPM) family proteins-NPM1, NPM2 and NPM3-expressed in oocytes are presumed to cooperatively regulate sperm chromatin remodeling. We characterized ...
متن کاملNucleoplasmin-mediated chromatin remodelling is required for Xenopus sperm nuclei to become licensed for DNA replication.
During late mitosis and early G(1), a series of proteins are assembled onto replication origins, resulting in them becoming 'licensed' for replication in the subsequent S phase. Four factors have so far been identified that are required for chromatin to become functionally licensed: ORC (the origin recognition complex) and Cdc6, plus the two components of the replication licensing system RLF-M ...
متن کاملRemodeling of sperm chromatin induced in egg extracts of amphibians.
Sperm nuclear basic proteins of Bufo japonicus consist of 2 distinct protamines, whereas those of Xenopus laevis consist of 6 sperm-specific basic proteins (SP1-6) in addition to H3, H4 and smaller amounts of H2A and H2B. Cloning of pertinent cDNAs and partial amino acid sequence studies suggested that these 6 sperm-specific proteins of Xenopus are encoded by 3 distinct genes. Despite differenc...
متن کاملHuman Sperm Aster Formation and Chromatin Configuration in Rabbit Oocytes Following Intracytoplasmic Sperm Injection Using a Piezo-Micromanipulator
In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...
متن کاملP-19: Association of Poor Chromatin Remodeling with Cytosolic ROS and Mitochondrial ROS in Sperm of Infertile Men
Background: Cytoplasm and mitochondria are considered as the major origins of sperm ROS production. Sperm is prone to DNA damage by exposure to ROS or due impaired chromatin remodeling or low DNA protamination. Therefore, the aim of this study was to see if there is any association between impaired chromatin packaging and origin of ROS production. Materials and Methods: Cytosolic ROS, mitochond...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1993
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1993.00059.x